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unclear. Here, we exploit the MSH1 system in Arabidopsis and tomato to introduce rootstock epigenetic variation to
grafting experiments. Introducing mutations dcl2, dcl3 and
dcl4 to the msh1 rootstock disrupts siRNA production and reveals RdDM targets of methylation repatterning.
Progeny from grafting experiments present enhanced development vigor
relative to controls. This heritable enhancement-by-grafting phenotype is RdDM-dependent, involving 1380 differentially methylated genes,
many inside auxin-associated gene pathways.
Growth vigor is related to robust root development of msh1 graft progeny,
a phenotype related to auxin transport primarily based on inhibitor assays.
Large-scale discipline experiments show msh1 grafting results on tomato plant performance,
heritable over 5 generations, demonstrating the agricultural potential of epigenetic variation. Plants display exceptional adaptability to diverse and
variable environments. -
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